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. 2019 May 4;9:e00093. doi: 10.1016/j.mec.2019.e00093

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 8 — Determination of the limiting factors for 6-MSA and m-cresol production. A) 6-MSA titers produced by strain JHY163 (ura3::^PpoptMSAS-^optnpgA) expressing additionally ^PpoptMSAS and ^optnpgA from multi-copy plasmid pJHV49 (pl. M; black) or as control the empty plasmid pRS42K (pl. Empty; light blue). B) m-cresol titers produced by strain JHY162 (ura3::^PpoptMSAS-^optnpgA-^optpatG) carrying additionally as a control the empty plasmid pRS42K (pl. Empty; red), or expressing ^PpoptMSAS,^optnpgA,^optpatG from pJHV53 (pl. M/patG; orange) or ^optpatG from pJHV13 (pl. patG; blue) and strain JHY163 (ura3::^PpoptMSAS-^optnpgA) (g. M) expressing additionally ^PpoptMSAS,^optnpgA,^optpatG from pJHV53 (pl. M/patG; purple) or ^optpatG from pJHV13 (pl. patG; grey). High-OD fermentations (starting OD = 5) were performed in biological duplicates at 30 °C in YPD supplemented with G418 (error bars represent standard deviations). g. M. indicates genomic expression of MSAS/npgA, g. M/patG indicates genomic expression of MSAS/npgA and patG, + pl. indicates additional overexpression from multi-copy plasmids. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)