. 2019 May 4;9:e00093. doi: 10.1016/j.mec.2019.e00093

Table 1.

Plasmids and yeast strains used in the present study. Genes from Saccharomyces cerevisiae (Sc), Penicillium patulum (Pp), Aspergillus nidulans (An), Aspergillus niger (Ani) Aspergillus clavatus (Ac), codon-optimized genes (opt) or variants previously used by Wattanachaisaereekul et al. (2008) (var) are indicated by prefixes in superscript. Other abbreviations: hphNT1: hygromycin resistance; Amp^r: ampicillin resistance; kanMX: kanamycin resistance. If not stated otherwise, promoters (p) were taken 1–500 bp upstream and terminators (t) 1–300 bp downstream of respective open reading frames.

Plasmid	Plasmid based on	Relevant features	Reference
pRS42K	–	2μ, kanMX, Amp^r	Taxis and Knop (2006)
pRS62H	–	2μ, hphNT1, Amp^r, HXT7p^{−1- −392}, FBA1t	Farwick et al. (2014)
pJHV1	pRS42K	2μ, kanMX, Amp^r, MET25p^{−1- −384}, CYC1t	This work
pJHV2	pRS62H	2μ, hphNT1, Amp^r, HXT7p^{−1 -−392}-^AnoptnpgA-FBA1t	This work
pJHV5	pJHV7	2μ, kanMX, Amp^r, PGK1p-^AniMSAS-CYC1t	This work
pJHV7	pRS42K	2μ, kanMX, Amp^r, PGK1p, CYC1t	This work
pJHV11	pJHV7	2μ, kanMX, Amp^r, PGK1p-^PpMSAS-CYC1t	This work
pJHV13	pJHV7	2μ, kanMX, Amp^r, PGK1p, CYC1t, FBA1p-^AcoptpatG-ADH1t	This work
pJHV17	pJHV7	2μ, kanMX, Amp^r, PGK1p-^PpvarMSAS-CYC1t	This work
pJHV20	pRS62H	2μ, hphNT1, Amp^r, HXT7p^{−1- −392}-^AnnpgA-FBA1t	This work
pJHV36	pJHV7	2μ, kanMX, Amp^r, PGK1p-^PpoptMSAS-CYC1t	This work
pJHV49	pJHV36	2μ, kanMX, Amp^r, PGK1p-^PpoptMSAS-CYC1t, HXT7p^{−1- −392}-^AnoptnpgA-FBA1t	This work
pJHV53	pJHV13	2μ, kanMX, Amp^r, PGK1p-^PpoptMSAS-CYC1t, HXT7p^{−1- −392}-^AnoptnpgA-FBA1t, FBA1p-^AcoptpatG-ADH1t	This work
pRCC-K_URA3	–	2μ, kanMX, Amp^r, ROX3p-^optCas9-CYC1t, pSNR52-gRNA for URA3	Mara Reifenrath, University of Frankfurt
SiHV33	–	ConLS′-gfp-dropout-ConRE′-KanMX-URA 3′Hom-KanR-ColE1-URA3 5′Hom	Simon Harth, University of Frankfurt
pRS426CTMSA-PP	–	2μ, URA3, Amp^r, TEF1p-^PpvarMSAS-CYC1t	Verena Siewers, Chalmers, Gothenburg
pDKP4832	–	2μ, URA3, Amp^r, GAL1p-^AnnpgA	Verena Siewers, Chalmers, Gothenburg
S. cerevisiae strain	Parent strain	Relevant features	Reference
CEN.PK2-1C	–	MATa leu2-3112 ura3-52 trp1-289 his3-Δ1 MAL2–8^cSUC2	Entian and Kötter (2007)
JHY162	CEN.PK2–1C	ura3::PGK1p-^PpoptMSAS-CYC1t_HXT7p^{−1- −392}-^AnoptnpgA-FBA1t_FBA1p-^AcoptpatG-ADH1t	This work
JHY163	CEN.PK2–1C	ura3::PGK1p-^PpoptMSAS-CYC1t_HXT7p^{−1- −392}-^AnoptnpgA-FBA1t	This work