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. 2019 Apr 28;15:274–281. doi: 10.1016/j.isci.2019.04.022

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© 2019 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Spindle Actin Inhibition Results in Mitotic Defects

(A) Image examples of synchronized RPE-1 cells, stably expressing the chromatin marker H2B-mCherry (red) and treated with indicated compounds at 00:00. Mitotic events are indicated by arrowheads. Scale bar, 25 μm; time stamp, h:min.

(B) Quantification of mitotic defects as shown in (A). Data are presented as mean + SD; n > 15 mitotic events, pooled from three independent experiments.

(C) RPE-1 cells, stably expressing the chromatin marker H2B-mCherry (red) were transfected with GFP or GFP-Arpin and synchronized at the G2/M border. Image examples show mitotic progression after release of the mitotic block. Mitotic events are indicated by arrowheads. Scale bar, 25 μm; time stamp, h:min.

(D) Quantification of mitotic defects as shown in (C). Data are presented as mean + SD; n > 7 mitotic events. Immunoblot indicates expression and molecular weight of Arpin.