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. 2019 Mar 13;8(1):73–94. doi: 10.1016/j.jcmgh.2019.03.001

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

CLPP overexpression and establishment of stable hepatoma cell lines. (A) Histogram plots showing percentage of GFP positivity in stable HepG2 and Huh7 cells expressing either empty GFP vector or CLPP-GFP as analyzed by flow cytometry. (B) Immunofluorescence images of stable cell clones of HepG2 and Huh7 cells showing expression of either GFP or CLPP-CLPP (green). Note that CLPP-GFP showed a punctate expression which co-localized with Mitotracker red. Nucleus was counterstained with DAPI. (C) Growth of cells expressing GFP and CLPP-GFP was monitored by uptake of crystal violet dye at specified time intervals and quantified by reading the absorbance at 570 nm. The data are represented as mean ± SD from 3 independent experiments.