Figure 3.

DPS-2 dramatically reduces cell viability of colon and melanoma cancer cell lines, while it affects cell viability of NIH3T3 and HepG2 cells in higher concentrations and shows very low toxicity in WJ-MSCs. (A) Cell Viability in RKO, HT29, and Colo-205 cell lines was measured using the SRB assay after 72-hour treatment with increasing concentrations of DPS-2 inhibitor. PLX4720 inhibitor was used as a positive control for cell viability reduction. (B) Light microscope images of 2D culture in RKO cell line after treatment with 2 μΜ, 5 μΜ, and 10 μΜ of DPS-2 inhibitor for 72 hours. (C) Cell Viability in Caco-2, HCT116, and DLD-1 cell lines was measured, using the SRB assay, after 72-hour treatment with increasing concentrations of DPS-2 inhibitor. Data are representative for two independent experiments, each of which was performed in duplicates. SD was used for error bar generation. (D) The effect of different concentrations (0-10 μM) of DPS-2 in five melanoma cell lines treated for 24 and 72 hours. All data are presented as the mean ± SD. (E) Percentage of cytotoxicity in WJ-MSCs cells after 48 hours of incubation with various concentrations of DPS-2 and respective survival curves (y = % viability, x = concentration μM). Cell viability was assessed using the MTS assay. Data are presented as mean of cell survival compared with negative control (cell survival assumed 100%). (F) Cell viability in NIH3T3 cell line was measured after 48-hour treatment with increasing concentrations of DPS-2 inhibitor. (G) Cell viability in HepG2 cell line was measured after 48-hour treatment with increasing concentrations of DPS-2 inhibitor. Cell viability was assessed using the MTS assay. Data are presented as mean of cell survival compared with negative control (cell survival assumed 100%). The 0.2% DMSO diluted in DMEM was used as a negative control for cell viability reduction. IC₅₀ values are given for each graph. Experiments were performed in triplicates. SD was used for error bar generation.