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Intracellular content (pmol/mg of protein) of thiamine (A, B, C) and ThDP (D, E, F) as a function of time after addition of 0 (control), 1, 10 and 50 μM thiamine (A, D), BFT (B, E) or 0 (control), 0.5, 5 and 25 μM SuBT (C, F). As the medium contained no serum, a small amount of thiamine (10 nM) was added to the thiamine-free DMEM medium at t = 0 in all samples. Please note the logarithmic scales in A, B and C. (Mean ± SD, n-3).
