Skip to main content
. 2019 Apr 13;11(4):222. doi: 10.3390/toxins11040222

Table 2.

Optimised LC-MS/MS conditions and calibration curve performances (R2) for target compounds in different matrices.

Compound Ionisation
Mode		 Target Ion RT
(min)		 Precursor
(m/z)		 NCE
(ev)		 Fish Feed (R2) Salmon (R2) Zebrafish (R2) ISTD
DON ESI neg [M+CH3COO]− 12.3 355.1387 17 0.9996 0.9964 0.9996 13C-DON
3-ADON ESI neg [M+CH3COO]− 23.8 397.1493 15 0.9998 0.9975 0.9999 13C-3-ADON
15-ADON ESI pos [M+Na]+ 23.7 361.1258 15 0.9999 0.9986 0.9969 13C-15-ADON
DON-3G ESI neg [M+CH3COO]− 15.1 517.1916 17 0.9993 0.9935 0.9851 13C-DON-3G
NIV ESI neg [M+CH3COO]− 5.30 371.1337 17 0.9983 0.9901 0.9972 13C-NIV
T-2 ESI pos [M+NH4]+ 28.0 484.2541 15 0.9995 0.9978 0.9995 13C-T-2
HT-2 ESI neg [M+CH3COO]− 26.4 483.2225 15 0.9998 0.9961 0.9998 13C-HT-2
OTA ESI neg [M−H]− 27.3 402.0739 32 0.9992 0.9984 0.9998 13C-OTA
ZEN ESI neg [M−H]− 29.5 317.1384 50 0.9999 0.9985 0.9998 13C-ZEN
Ergonovine ESI pos [M+H]+ 21.9 326.1863 50 0.9996 0.9992 0.9999 MetErg
Ergosine ESI pos [M+H]+ 27.6 548.2868 27 0.9990 0.9979 0.9999 BromCri
Ergotamine ESI pos [M+H]+ 28.0 582.2711 32 0.9973 0.9985 0.9999 BromCri
Ergocornine ESI pos [M+H]+ 28.1 562.3024 25 0.9992 0.9973 0.9998 BromCri
α-Ergocryptine ESI pos [M+H]+ 28.8 576.3180 25 0.9993 0.9980 0.9999 BromCri
Ergoscristine ESI pos [M+H]+ 29.0 610.3024 27 0.9984 0.9980 0.9999 BromCri
ENN A ESI pos [M+NH4]+ 35.1 699.4903 27 0.9943 0.9981 0.9992 -
ENN A1 ESI pos [M+NH4]+ 34.4 685.4746 27 0.9984 0.9987 0.9991 -
ENN B ESI pos [M+NH4]+ 33.1 657.4433 27 0.9986 0.9952 0.9998 -
ENN B1 ESI pos [M+NH4]+ 33.8 671.4590 27 0.9993 0.9987 0.9993 -
Daidzein ESI neg [M−H]− 26.1 253.0506 75 0.9993 0.9980 0.9982 -
Daidzin ESI neg [M+CH3COO]− 23.1 475.1246 10 0.9997 0.9984 0.9998 -
Genistein ESI neg [M−H]− 27.3 269.0455 70 0.9997 0.9986 0.9979 -
Genistin ESI neg [M+CH3COO]− 24.4 491.1195 10 0.9994 0.9974 0.9999 -
Glycitein ESI neg [M−H]− 26.4 283.0612 35 0.9998 0.9997 0.9989 -
Glycitin ESI neg [M+CH3COO]− 23.6 505.1351 10 0.9994 0.9979 0.9994 -