Figure 2.
(A,B) Five-week-old female BALB/c mice were fed BA (control diet) or BD diet for 12 weeks. Epidermal langerhans cells (LCs) were identified as CD45+IA/IE+ cells. Three mice for each group were analyzed. (C) ATP release in response to BAC from the ear skins of BA and BD mice at 12 weeks after the initiation of BA and BD diets. Five mice of each group were analyzed. (D) ACD (left panel) and ICD (right panel) in BA and BD mice at 12 weeks after the initiation of BA and BD diets. Five mice of each group were analyzed. (E,F) ears of BA and BD mice elicited irritant contact dermatitis (ICD) response at 24 h after CrO application. (G) mRNA expression of molecules associated with LC differentiation and survival in KCs. Data are representative of 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.