Table 2.

Amino acid differences in Env V4 among different EIAV strains detected using single genome amplification (SGA).

	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	Mutation Rate (236D/-)
	2	2	2	2	2	2	2	2	2	3	3	3	3	3	3	3	3	3	3	4	4	4	4	4	4	4	4	4	4	5	5	5
	1	2	3	4	5	6	7	8	9	0	1	2	3	4	5	6	7	8	9	0	1	2	3	4	5	6	7	8	9	0	1	2
EIAVLN40	N86	A88	I88	T88	I88	L88	V88	P88	E85	V87	E88	M88	Q87	Q88	S80	D72	N71	N88	T88	W88	I88	P88	K87	R80	C88	N86	E87	T87	W88	A88	R81	V88	17.0%
	S2								V1	I1			H1		R8	-15	K14						N1	G8		K2	K1	I1			K7
									K1							N1	H1
									Q1
EIAVDLV34	N51	A97	I98	T98	I98	L97	V98	P97	E94	V60	E96	M98	Q98	Q82	S86	D63	N75	N98	T98	W95	I95	P97	K96	R57	C97	N85	E79	T96	W94	A94	R75	V94	30.6%
	S47	T1				V1		L1	K3	I38	K2			K8	R10	-30	K22			S1	S2	L1	Q1	G41	W1	K10	K17	A1	C3	C2	K20	I4
									D1					R8	K1	N5	Y1			C2	C1		P1			S3	G2	I1	R1	S1	T3
															N1															T1
EIAVDLV121	N76	A95	I95	T92	I95	L95	V94	P95	E92	I10	E93	M94	Q94	Q91	R54	-74	K69	N95	T95	W95	I95	P95	K95	R68	C95	N74	E83	T94	W95	A93	K55	V95	77.9%
	S18			A3			I1		K1	V84	K1	V1	H1	R3	S40	D19	N23							G25		K21	K11	A1		T2	R40
	K1								D1	L1	V1			K1	N1	N2	S2							K2			G1
									Q1								E1
pLGFD3-8	N100	A100	I100	T100	I100	L100	V100	P100	E99	V97	E99	M100	Q98	Q100	S87	-100	K100	N100	T97	W98	I99	P100	K100	G88	C99	K99	K99	T100	W99	A100	R97	V100	100%
									Q1	I3	K1		H1		R13				I3	C2	V1			R12	G1	N1	E1		C1		K2
													R1																		M1

The additional amino acid D, which consistently appeared in virulent EIAV strains, is marked with dark grey, and N-linked glycosylation sites are marked with light grey.