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. 2019 Mar 29;11(4):446. doi: 10.3390/cancers11040446

Figure 6.

Figure 6

A-375 cells under KCNN2-siRNA and ammonium nitrate treatment. (A) Miconazole effect in A-375 cells transfected with siRNA-KCNN2 and non-specific siRNA control. Bars without miconazole (left end of the graph) are expressed as % of untreated cells (NT); bars with miconazole (right end of the graph) are expressed as % of the corresponding control (NT with miconazole vs. NT without miconazole; siRNA Asp with miconazole vs. siRNA Asp without miconazole; siRNA KCNN2 with miconazole vs. siRNA KCNN2 without miconazole). Miconazole anti-proliferative effect is almost doubled in siRNA KCNN2-treated cells as compared to siRNA non-specific-treated cells. (B) KCNN2 protein expression in siRNA treated cells. siRNA KCNN2 strongly reduces KCNN2 expression in A-375 cells. (C) Ammonium nitrate has no anti-proliferative effect on A-375 cells; therefore, we may conclude that the anti-proliferative action of miconazole-nitrate is due to miconazole. * indicate p < 0.01.