Figure 2.

Rescue of the TGEV-GFP infectious clone in PK-15 cells. (A) PK-15 cells were infected with the recombinant or parental virus or mock infected, and at 24 h post-infection, their green fluorescence was visualized. (B) CPE or green fluorescence of PK-15 cells infected with the recombinant viruses TGEV-GFP or parental TGEV or mock infected was visualized at 48 h post-infection. (C) The expression of the TGEV N protein in PK-15 cells infected with recombinant TGEV-GFP or mock infected was analyzed by Western blotting using rabbit poly-antiserum against TGEV N protein. (D) The mutation of A4553T at nt 4553 was determined by sequencing after performing RT-PCR in triplicate.