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Details of the oligonucleotide primers used to produce the specific sgRNA for the double digestion of targeted BAC.
| Primer | Sequence |
|---|---|
| ssDNAa-F | TTAATACGACTCACTATA GGCTCCACAAAATCAATTGA GTTTTAGA GCTAGA |
| ssDNAb-F | TTAATACGACTCACTATA GGTCTTGGTATGAAGCGTAG GTTTTAGA GCTAGA |
| ssDNA-R | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAA CGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
