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. 2019 Apr 24;17(4):244. doi: 10.3390/md17040244

Figure 5.

Figure 5

Figure 5

AATP abolishes vasculogenic mimicry (VM) formation and decreases vascular endothelial growth factor (VEGF) secretion in HT1080 cells. (a) Cells were seeded on Matrigel pre-coated 96-well plates and incubated in medium containing 50 and 100 μM AATP for 3 h. Then, the photographs of VM formation were taken with an inverted microscope and analyzed by imageJ. (b) The cells pre-treated with indicated concentrations AATP for 1 h were stimulated with 100 μM CoCl2 for 24 h. The level of VEGF secretion was detected using ELISA kit. AATP inhibits hypoxia-induced expression of HIF-1α and blocks AKT/mTOR/p70S6K signaling in HT1080 cells. (c, d) Cells were incubated with 20, 50 and 100 μM AATP for 1 h and stimulated with 100 μM CoCl2 for 24 h. The expression of HIF-1α, p-AKT/AKT, p-mTOR/mTOR and p-p70S6K/p70S6K were determined by western blotting and β-actin was used as loading controls. # p < 0.001 vs. untreated control, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. CoCl2 stimulation.