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. 2019 Mar 29;17(4):199. doi: 10.3390/md17040199

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Purification and characterization of the antifungal agents produced by Bacillus sp. CS30 against M. grisea. (A) HPLC chromatogram of the antifungal agent produced by Bacillus sp. CS30. (B) Growth inhibition of M. grisea caused by the purified antifungal agents. Sterile filter paper disks containing the same amount of purified active agents and a methanol control are indicated. The active agents eluted at 41.2 min and 42.7 min in panel A are indicated as “eluent 41.2 min” and “eluent 42.7 min” in panel B, respectively.