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. 2019 Apr 22;11(4):233. doi: 10.3390/toxins11040233

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The native molecular weights of the PirA^vp, PirB^vp and PirA^vp/PirB^vp complex were estimated by gel filtration analysis. (A,B) Compared to PirA^vp and PirB^vp, the PirA^vp/PirB^vp complex has a larger molecular weight and thus appeared in a smaller elution volume. (C) The proteins provided in the gel filtration calibration kits were used to create a plot of Kav against log MW. Using this standard curve, the molecular weights of PirA^vp, PirB^vp and the PirA^vp/PirB^vp complex were calculated. A, C, O, CA, and R represented aldolase (158 kDa), conalbumin (75 kDa), ovalbumin (43 kDa), carbonic anhydrate (29 kDa), and ribonuclease A (13.7 kDa), respectively.