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. 2019 Apr 9;11(4):335. doi: 10.3390/v11040335

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antibody-mediated DENV2 infection inhibition assay. Vero cells were either treated with various concentrations of rabbit polyclonal anti-annexin II antibody, 20 µg of rabbit polyclonal anti-histag antibody or no antibody (control), for 1 h, prior to DENV2 adsorption. Infections were allowed for 30 h. (A) Intracellular DENV2 titer was quantified with Liferiver DENV general-type real time RT-PCR Kit. (B) Extracellular DENV2 titer was determined by TCID₅₀. The experiment was independently repeated in triplicates. Statistically significant reduction in virus infection and output were observed in the anti-annexin-II-treated Vero cells, compared to the control-antibody-treated and no-antibody-treated Vero cells (** indicates p < 0.01, **** indicates p < 0.0001), as determined by one-way ANOVA analysis; ab—antibody.