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. 2019 Apr 4;11(4):782. doi: 10.3390/nu11040782

Figure 6.

Figure 6

B. adolescentis strain-stimulated RAW264.7 macrophages show active chromosome remodeling related to targeted gene promoter combination with transcriptional factors NF-κB and others, and IL-10 production. Macrophages were stimulated with B. adolescentis IF1-11, B. adolescentis IF1-03 or left untreated for 3 h (control) and the level of H3K9 acetylation on sites of transcription factor–genomic DNA interaction sequence at the Il10 proximal promoter locus was determined by qPCR with specific primers (Table 1). (A) Sp1, (B) C/EBPβ, (C) CREB, (D) NF-κB, (E) MAF, and (F) IL-10 yield in macrophages treated with B. adolescentis strains, and inhibition of H3K9 acetylation with C646. IL-10 was assayed by ELISA. Data are shown as mean ± SD for three independent experiments (* p < 0.05, ** p < 0.01). Sp1: Specific protein 1, C/EBPβ: CCAAT/enhancer binding protein β, CREB: c-AMP responsive element binding protein, NF-κB: Nuclear factor κB.