Figure 2.

Cumulative mortalities of Exopalaemon carinicauda in the challenge test. IM group, prawns were challenged with filtrated viral suspension via intermuscular injection; PO group, prawns were fed with tissues of DIV1-infected P. vannamei; CIM group, prawns were injected with sterile PPB-His buffer; CPO group, prawns were fed with commercial feed. Cumulative mortalities are shown as means of data from three replicates for each experimental group (each replicate contained 10 individuals). The mean points with same color indicate no significant difference (P > 0.05), and the mean points with different colors indicate a significant difference (P < 0.05). Overall analysis indicated there are very significant differences among IM, PO, and the two controls (P < 0.01). The curves were drawn based on the nonlinear regression following the three-parameter sigmoid equation.