Fig. 4. Western blot analysis of HO-1 protein levels in HSC-3 (A and C) and HaCaT cells (B and D) under different experimental conditions. Cells were treated with 75 μM and 200 μM of cisplatin for 4 h in the presence and absence of AuNCs. Cells not exposed to AuNCs and cisplatin served as a negative control. The treated and untreated cells were lysed and cell extract subjected to western blots with anti-HO-1 antibodies. The α-GAPDH used as a loading control.
