Table 1.
Peptides identified by HPLC-MS/MS from Fr41‒44 collected by RP-HPLC of Ulva sp. protein hydrolysates with papain. The Q-values are an estimation of the bitterness of the peptides and are calculated and summarised in the last column.
| Samples | Amino Acid Sequences | Modifications | Observed MW |
Calculated MW |
Observed m/z |
Theoretical m/z |
Charge (+) |
Database | Accessions | Q-Values |
|---|---|---|---|---|---|---|---|---|---|---|
| Fr41 | TGGSLHAA | 712.41 | 712.35 | 357.21 | 357.18 | 2 | NCBInr | XP_005851095.1; EFN58993.1 | 607.5 | |
| VVPKAAPPPN | 988.76 | 988.57 | 495.39 | 495.29 | 2 | NCBInr | XP_005843189.1; EFN51087.1 | 1343 | ||
| ATKPAN | 600.41 | 600.32 | 301.21 | 301.17 | 2 | NCBInr | XP_001696486.1; XP_001696477.1 | 1001.67 | ||
| SGAASASGAA | 748.40 | 748.34 | 375.21 | 375.17 | 2 | NCBInr | RRRRRKXZ51346.1 | 377 | ||
| HSAVFAAS | 788.51 | 788.38 | 395.26 | 395.20 | 2 | NCBInr | GAX77766.1 | 883.75 | ||
| NGNAASPGQPPLEVH | 1486.84 | 1486.72 | 744.43 | 744.37 | 2 | NCBInr | RRRRRJAC80986.1 | 960 | ||
| HQWGGAPQH | 1016.69 | 1016.46 | 509.35 | 509.24 | 2 | NCBInr | XP_002500034.1; ACO61292.1 | 794.44 | ||
| KFKGMNHINDIEKFK | 1847.96 | 1847.97 | 616.99 | 617.00 | 3 | UniProt | sp|B9DRW5 | 1304 | ||
| GAVHAMVDTAKIHKGKK | 1789.95 | 1790.00 | 597.66 | 597.67 | 3 | UniProt | RRRRRsp|Q3TC72 | 1048.23 | ||
| Fr42 | AGGPNQPPN | 850.48 | 850.39 | 426.25 | 426.20 | 2 | NCBInr | XP_001694488.1; EDP02483.1 | 941.11 | |
| AANITVPAAN | 940.58 | 940.50 | 471.30 | 471.26 | 2 | NCBInr | XP_002957564.1; EFJ41334.1 | 1062 | ||
| DSLSAIGGAPDG | 1058.45 | 1058.49 | 530.23 | 530.25 | 2 | NCBInr | JAC65094.1 | 885.83 | ||
| SAGVLPWK | 856.50 | 856.48 | 429.26 | 429.25 | 2 | NCBInr | KXZ46138.1 | 1500 | ||
| DGLIDGL | 694.35 | 701.36 | 695.36 | 702.37 | 1 | UniProt | tr|A0A1C9C803|A0A1C9C803_9FLOR | 1270 | ||
| GDLIDVA | 694.35 | 701.36 | 695.36 | 702.37 | 1 | UniProt | tr|M1VAH3|M1VAH3_CYAM1 | 1270 | ||
| NQVTNLIVA | 970.46 | 970.54 | 486.24 | 486.28 | 2 | UniProt | tr|A0A1G4NVL3|A0A1G4NVL3_9FLOR | 1091.11 | ||
| GSASGAFVY | 857.46 | 857.39 | 429.74 | 429.70 | 2 | UniProt | tr|M1V5F7|M1V5F7_CYAM1 | 972.22 | ||
| Fr43 | HGPPPPSPYRSAAGRAAL | 1800.90 | 1800.94 | 601.31 | 601.32 | 3 | NCBInr | XP_005846003.1; EFN53901.1 | 1297.22 | |
| EAEPAEAA | 786.37 | 786.34 | 394.19 | 394.18 | 2 | NCBInr | XP_005852249.1; EFN60147.1 | 898.75 | ||
| SIAGVAAFIG | 904.48 | 904.50 | 453.25 | 453.26 | 2 | NCBInr | JAC64130.1 | 1251 | ||
| YAAKMRK | 866.54 | 866.48 | 434.28 | 434.25 | 2 | NCBInr | XP_007512502.1; CCO17102.1 | 1337.14 | ||
| DDLKGTF | 794.38 | 794.38 | 398.20 | 398.20 | 2 | NCBInr | XP_003058074.1; EEH58025.1 | 1155.71 | ||
| GGVYGTSAR | 866.50 | 866.42 | 434.26 | 434.22 | 2 | NCBInr | XP_001700029.1; EDP07725.1 | 722.22 | ||
| GSECMWFAS | 1016.33 | 1016.37 | 509.17 | 509.19 | 2 | NCBInr | XP_001693035.1; EDP03604.1 | 923.33 | ||
| AGFSYFGESS | 1050.45 | 1050.43 | 526.23 | 526.22 | 2 | NCBInr | XP_002951597.1; EFJ47408.1 | 957 | ||
| DLLALRELDVACN | 1443.77 | 1443.74 | 722.89 | 722.88 | 2 | NCBInr | JAT73014.1 | 1167.69 | ||
| NGGDLPGAL | 812.42 | 812.40 | 407.22 | 407.21 | 2 | NCBInr | XP_003055625.1; EEH60877.1 | 968.89 | ||
| ALLQQQAQMAAALPLPP | 1759.90 | 1759.97 | 587.64 | 587.66 | 3 | NCBInr | XP_005847720.1; EFN55618.1 | 1299.41 | ||
| LTPCAVPE | 828.43 | 828.41 | 415.22 | 415.21 | 2 | NCBInr | KXZ44026.1 | 1383.75 | ||
| ERTGRVAM | 918.48 | 918.47 | 460.25 | 460.24 | 2 | NCBInr | KXZ43890.1 | 771.25 | ||
| LNCALK | 660.43 | 660.36 | 331.22 | 331.19 | 2 | NCBInr | XP_001416982.1; ABO95275.1 | 1176.67 | ||
| GAAPTPPSPPPATKPSTPPKPPT | 2190.15 | 2190.17 | 731.06 | 731.06 | 3 | NCBInr | XP_002958348.1; EFJ40570.1 | 1558.69 | ||
| IECCLLFALV | 1122.55 | 1122.58 | 562.28 | 562.30 | 2 | NCBInr | GAX82307.1 | 1585 | ||
| PVGCLPK | 712.40 | 712.39 | 357.21 | 357.20 | 2 | NCBInr | JAC75907.1 | 1550 | ||
| DEDESSFGK | 1012.47 | 1012.40 | 507.24 | 507.21 | 2 | UniProt | tr|R7QMS5|R7QMS5_CHOCR | 712.22 | ||
| GASPVTFVFT | 1024.49 | 1024.52 | 513.25 | 513.27 | 2 | UniProt | tr|M1VHB3|M1VHB3_CYAM1 | 1295 | ||
| AGDLGAYG | 722.39 | 722.32 | 362.20 | 362.17 | 2 | UniProt | tr|A0A1X6NU59|A0A1X6NU59_PORUM | 911.25 | ||
| RSARVRVGSTAT | 1259.69 | 1259.71 | 630.85 | 630.86 | 2 | UniProt | tr|A0A1X6NKL7|A0A1X6NKL7_PORUM | 665.83 | ||
| INNNKIITNL | Deamidated (N)@2 | 1156.65 | 1156.65 | 579.33 | 579.33 | 2 | UniProt | tr|A0A1Z1MP80|A0A1Z1MP80_9FLOR | 1323 | |
| DAVEIWRVK | 1114.58 | 1114.61 | 558.30 | 558.31 | 2 | UniProt | tr|R7QMS0|R7QMS0_CHOCR | 1488.89 | ||
| Fr44 | DEVIPGAL | 812.43 | 812.43 | 407.22 | 407.22 | 2 | NCBInr | GAX84002.1 | 1440 | |
| DATFCGDLDDA | 1141.54 | 1141.42 | 571.78 | 571.72 | 2 | NCBInr | XP_001697003.1; EDP00695.1 | 830 | ||
| PKPPALCN | 838.47 | 838.44 | 420.24 | 420.23 | 2 | NCBInr | KXZ44308.1 | 1562.5 | ||
| PPNPPNPPN | 942.56 | 942.46 | 472.29 | 472.24 | 2 | NCBInr | XP_002955492.1; EFJ43345.1 | 1743.33 | ||
| TPALVSQLH | 964.50 | 964.53 | 483.26 | 483.27 | 2 | NCBInr | XP_011396301.1; KFM23431.1 | 1195.56 | ||
| TMSDRFL | 868.51 | 868.41 | 435.26 | 435.21 | 2 | NCBInr | XP_002499460.1; ACO60718.1 | 1160 | ||
| AAGAAPLL | 682.44 | 682.40 | 342.23 | 342.21 | 2 | NCBInr | AAW51128.1 | 1297.5 | ||
| LIKELDSN | 930.62 | 930.50 | 466.32 | 466.26 | 2 | UniProt | tr|A0A1Z1MG56|A0A1Z1MG56_9FLOR | 1303.75 |