Table 4.
Ingenuity Pathway Analysis of differentially expressed genes from the fetal sheep microglia whole transcriptomes analysis.
| Comparison | # genes | Top 10 signaling pathways | -log(p-value) |
|---|---|---|---|
| LPS100 vs. Control | 1779 | Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis | 16.7 |
| Dendritic Cell Maturation | 12.9 | ||
| iNOS Signaling | 12.6 | ||
| Role of IL-17A in Arthritis | 12.5 | ||
| IL-10 Signaling | 11.8 | ||
| Role of Tissue Factor in Cancer | 11.6 | ||
| Production of Nitric Oxide and Reactive Oxygen Species in Macrophages | 11.1 | ||
| Neuroinflammation Signaling Pathway | 10.9 | ||
| Toll-like Receptor Signaling | 10.7 | ||
| CD40 Signaling | 10 | ||
| LPS100+A10 vs. Control | 4721 | Molecular Mechanisms of Cancer | 14.1 |
| Colorectal Cancer Metastasis Signaling | 11.2 | ||
| Toll-like Receptor Signaling | 11 | ||
| NF-κB Signaling | 10.6 | ||
| Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis | 10.4 | ||
| PI3K Signaling in B Lymphocytes | 10.4 | ||
| Role of Tissue Factor in Cancer | 10.3 | ||
| B Cell Receptor Signaling | 9.83 | ||
| TREM1 Signaling | 7.83 | ||
| Protein Kinase A Signaling | 7.81 | ||
| LPS100+B100 vs. Control | 4049 | Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis | 13.6 |
| PI3K Signaling in B Lymphocytes | 11.4 | ||
| Production of Nitric Oxide and Reactive Oxygen Species in Macrophages | 10.2 | ||
| Molecular Mechanisms of Cancer | 10.2 | ||
| Activation of IRF by Cytosolic Pattern Recognition Receptors | 10.2 | ||
| IL-10 Signaling | 9.63 | ||
| B Cell Receptor Signaling | 9.55 | ||
| iNOS Signaling | 9.21 | ||
| Role of Tissue Factor in Cancer | 9.06 | ||
| Toll-like Receptor Signaling | 8.93 | ||
| LPS100+A10 vs. LPS100 | 8 | ||
| LPS100+B100 vs. LPS100 | 0 | ||
| LPS100+A10 vs. LPS100+B100 | 1132 | T Cell Exhaustion Signaling Pathway | 5.46 |
| Dendritic Cell Maturation | 5.43 | ||
| Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis | 5.42 | ||
| Altered T Cell and B Cell Signaling in Rheumatoid Arthritis | 5.22 | ||
| Th17 Activation Pathway | 5.16 | ||
| Leukocyte Extravasation Signaling | 4.81 | ||
| Superpathway of Cholesterol Biosynthesis | 4.8 | ||
| IL-10 Signaling | 4.49 | ||
| Role of Tissue Factor in Cancer | 4.31 | ||
| Th1 and Th2 Activation Pathway | 4.26 |
Differential analysis of count data was done with the Bioconductor limma package. Differentially expressed genes were selected, based on a 1.5-fold change and an FDR < 0.05. Up regulation and down regulation represent positive and negative log2 fold changes, respectively. For details on “raw gene” level, see our GitHub repository or directly here. Bold font highlights signaling pathways common with astrocytes. Orange: positive z-score; blue: negative z-score. For further details as raw data and visualized activity patterns see GitHub.
Significant genes in microglia cultures at an FDR < 0.05 and a 1.5-fold change sorted on log(p-value).