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. 2019 Apr 19;17(4):234. doi: 10.3390/md17040234

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of 3-bromo-4,5-dihydroxybenzaldehyde (3-BDB) on the levels and bioactivity of heme oxygenase-1 (HO-1) in a concentration- and time-dependent manner. HaCaT cells were pretreated with 3-BDB and incubated for 24 h with the indicated concentrations. (a,c) RT-PCR and Western blotting (WB) were used to analyze the HO-1 mRNA and protein expression. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control. (b,d) The amount of bilirubin formed was used as an indicator, to assess HO-1 activity. * Significant difference compared to the control (p < 0.05).