Figure 1.

(A) Dose-dependent survival rate (MTS assay) of human embryonic stem cells (hESC) at 8 h shows decrease of cell survival to 60% at doses of 10 ppm (n = 6). (B) Representative bright field micrographs of hESC colonies treated with vehicle (ethanol) or OTA (5 and 10 ppm). White arrows indicate surviving cells and black arrows indicate dead cells. Micrographs show the suitable aspect and shape of surviving cells. White asterisks indicate the area from which the photographs were taken. (C) Number of attached cells after 6 and 24 h in two experimental groups compared to control. Magnification times: 20×.