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. 2019 Apr 15;17(4):227. doi: 10.3390/md17040227

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Autophagy of kidney cells CTX mediated was reduced by PPTT in mice. Kidneys from mice exposed to CTX and/or PPTT were subjected to make paraffin sections with routine methods. LC 3 or beclin-1 protein was probed with the corresponding first antibody and the fluorescein labeled secondary antibody and was then observed under an inverted fluorescence microscope, and photos were taken. The green color indicates the antibody and the blue color indicates the nucleus. Note: Significant difference is represented with # and *, p < 0.05; extremely significant difference with ## and **, p < 0.01. In addition, ## and # stand for difference versus normal group; ** and * for difference versus the CP group.

Autophagy of kidney cells CTX mediated was reduced by PPTT in mice. Kidneys from mice exposed to CTX and/or PPTT were subjected to make paraffin sections with routine methods. LC 3 or beclin-1 protein was probed with the corresponding first antibody and the fluorescein labeled secondary antibody and was then observed under an inverted fluorescence microscope, and photos were taken. The green color indicates the antibody and the blue color indicates the nucleus. Note: Significant difference is represented with # and *, p < 0.05; extremely significant difference with ## and **, p < 0.01. In addition, ## and # stand for difference versus normal group; ** and * for difference versus the CP group.