. 2019 Apr 15;11(4):849. doi: 10.3390/nu11040849

Table 13.

Expression changes of FGF23/Klotho-associated gene using quantitative real-time polymerase chain reaction in the kidney in the study of moderate Ca supplementation exposure to different diet Cd (Exp 2).

Cd (mg/kg Diet)	Klotho (Fold Change)			Fgfr1 (Fold Change)			Napi2a (Fold Change)
	Ca Supplementation		p	Ca Supplementation		p	Ca Supplementation		p
	−	+	p	−	+	p	−	+	p
0	1.00 ± 0.10	--	--	1.00 ± 0.20	--	--	1.00 ± 0.25	--	--
1	2.07 ± 0.77 *	0.54 ± 0.14	<0.001 ^#	2.07 ± 1.32	1.51 ± 0.90	0.282	4.44 ± 2.89 *	1.31 ± 1.21	0.008 ^#
5	24.64 ± 9.07 *	21.88 ± 6.89	0.453 ^#	1.90 ± 0.93	2.84 ± 0.89	0.032 ^#	2.82 ± 1.15 *	1.52 ± 0.83	0.009 ^#
50	6.91 ± 1.01 *	3.28 ± 1.09	<0.001 ^#	2.94 ± 0.85 *	4.04 ± 1.43	0.05 ^#	2.00 ± 0.95 *	1.17 ± 0.68	0.037^#
p	<0.001 **			0.001 **			<0.001 **
Cd (mg/kg Diet)	Cyp24a1 (Fold Change)			Cyp27b1 (Fold Change)
	Ca Supplementation		p	Ca Supplementation		p
	−	+	p	−	+	p
0	1.00 ± 0.10	--	--	1.00 ± 0.34	--	--
1	2.11 ± 1.57 *	1.23 ± 1.04	0.156	1.61 ± 0.87 *	11.99 ± 3.79	<0.001 ^#
5	0.57 ± 0.43 *	0.72 ± 0.48	0.471	0.52 ± 0.82 *	0.83 ± 0.49	0.318
50	0.36 ± 0.21 *	1.76 ± 1.36	0.01^#	0.44 ± 0.33 *	4.80 ± 1.31	<0.001 ^#
p	<0.001 **			0.001 **

Data are expressed as fold changes (mean ± SD, n = 10), normalized to GAPDH mRNA expression, where the values for the control mice were set at 1.0. Fold change = 2^−ΔΔCt; ΔCt = Ct_{target gene}−Ct_GAPDH; ΔΔCt = ΔCt_{targe group}−ΔCt_control. Data are mean ± SD (n = 10). ** p < 0.05 by ANOVA; # p < 0.05 by t test; * p < 0.05, vs. Fgfr1, fibroblast growth factor receptor 1.