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. 2019 Apr 8;11(4):502. doi: 10.3390/cancers11040502

Figure 6.

Figure 6

CM37 increases reactive oxygen species (ROS) levels in OC cells. (A) Representative flow cytometry for total intracellular ROS levels in OVCAR5 cells treated with DMSO or 1 µM CM37. (B–D) Fold change in ROS levels measured by flow cytometry in SKOV3, OVCAR3, and OVCAR5 treated with DSMO or 1 µM CM37 for 1 h. (E,F) Sphere formation in OVCAR5 cancer cells treated with 100 nM CM37 +/− 50 µM trolox for three days. Spheres were photographed under an inverted microscope (E) and counted (five fields per well; (F). Bars represent averages of triplicate measurements; * corresponds to p < 0.05. (G,H) Western blot measures γH2AX, H2AX, and β-actin protein levels in OVCAR5 and OVCAR3 cells cultured as spheres for six days and treated with 5 µM CM37 for 45 min, after 1 h or not of pre-treatment with Trolox (20 µM).