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. 2019 Apr 6;11(4):206. doi: 10.3390/toxins11040206

Figure 3.

Figure 3

Effect of co-expression of putative antitoxins with its cognate toxin on the growth of E. coli or E. amylovora. The growth of E. amylovora strain CTBT3-1 harboring (A) pEVS-cbtA or pEVS-cbeA-cbtA; E. coli strain BL21 (DE3) harboring (B) pBAD-parE or pBAD-rhh-parE, (C) pBAD-doc or pBAD-phd-doc, (D) pDEST-GNAT or pDEST-xre-GNAT was monitored. Analysis of strains carrying pDEST527 and pEVS143 plasmids was performed under IPTG inducible (+IPTG) or non-inducible conditions (-IPTG) while analysis of those carrying pBAD33 plasmids was performed under arabinose inducible (+ara) or non-inducible (-ara) conditions of expression. Bacterial growth is presented on a linear scale to allow visualization of moderate or mild effects of toxin expression on bacterial growth. Data points and error bars represent the means and standard deviations of three biological replicates.