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. 2019 Apr 8;11(4):498. doi: 10.3390/cancers11040498

Figure 5.

Figure 5

HBE1 expression is negatively regulated by the depletion of BCL11A expression. (A) Heatmap of differentially expressed HBE1 suppressor candidate genes (B) qRT-PCR confirmation of a candidate HBE1 suppressor gene selected based on RNA-seq analysis. * p < 0.05, ** p < 0.01. (C) Cells were transfected with a pCMV6-BCL11A-overexpression vector in a vector DNA concentration-dependent manner and changes in the mRNA expression level of BCL11A (left) and HBE1 (right) were analyzed by qRT-PCR. ** p < 0.01 compared to control cells. (D) Cells were transfected with control or BCL11A siRNA and exposed to gamma-irradiation (60 and 120 Gy). HBE1 mRNA levels were assessed by qRT-PCR. * p < 0.05, ** p < 0.01 compared to non-irradiated cells. (E) Representative image of clonogenic assay cells stained with crystal violet (left panel) and a graph showing mean survival rate as a function of radiation for negative control- or siBCL11A-treated cells. * p < 0.05 compared to control cells.