Skip to main content
. 2019 Apr 3;11(4):466. doi: 10.3390/cancers11040466

Figure 1.

Figure 1

Two-dimensional fluorescence difference gel electrophoresis (DIGE) proteomics analysis identified 22 protein spots significantly altered by 20 mM lithium chloride (LiCl) treatment of glioblastoma (GBM) cells for 24 h. The experiment was performed in triplicate. (a) Representative Cy3/Cy5 stained (presented in gray) gel showing differentially regulated spots after 24 h treatment of U251 cells with 20 mM LiCl. (b) The top six targets were excised from the gel based on significance after analysis of alterations in fluorescence intensity post-LiCl treatment and identified by mass spectrometry peptide analysis.