Figure 7.

The 5-FU-induced expression of p53 and p21 as well as the inhibitory effect of RSK and Akt inhibitors in KRAS(G13D)-mutated HCT116 cells are YB-1 dependent. KRAS(G13D)-mutated HCT116 cells were transfected with either 50 nM control siRNA or YB-1 siRNA. Twenty-four hours after transfection, cells were treated with and without 5-FU, LJI308 and MK2206 for 72 h, and protein samples were isolated. Phosphorylation and expression of the indicated proteins were analyzed by Western blotting. All densitometry values were obtained from the ratio of the indicated proteins to actin. The values for YB-1 were normalized to nontreated siRNA-transfected conditions. The ratio of YB-1 to actin was normalized to the nontreated control siRNA-transfected cells.