FIGURE 2.

Dopamine-induced D-aspartate release is mediated by EAATs. Retinal cultures were incubated for 90 min with [³H] D-aspartate (1 μCi/mL), washed and processed for release experiments as described in section “Materials and Methods.” Cultures were pre-incubated with the intracellular Ca²⁺ chelator, BAPTA-AM (50 μM; A) or the selective non-transportable inhibitor of EAATs DL-TBOA (100 μM; B) for 10 min. Then, cultures were incubated with DA (50 μM; A,B) for an additional period of 10 min. The results represent the mean ± SEM of four independent experiments. Statistical analyses were performed using one-way ANOVA followed by the Bonferroni post-test. **p < 0.01 (relative to basal); ^{$\mathrm{\#}\mathrm{\#}$}p < 0.01 (relative to DA). ^§ Not statistically different compared with DA. DA, Dopamine.