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. 2019 Apr 23;11(4):372. doi: 10.3390/v11040372

Figure 2.

Figure 2

Validation of protein-protein interactions between EBOV NP and RUVBL1 and RUVBL2. (A) RNase treatment was confirmed using agarose gel electrophoresis on samples that were left untreated or treated with RNase (designated by − RNase or +RNase). (B) Confirmation of HA-NP and FLAG-RUVBL1 interaction in the absence or presence of RNase. Whole cell lysates (WCL) samples were run in parallel with immunoprecipitations (IP) samples to confirm appropriate protein expression. (C) Reciprocal validation of protein-protein interaction between HA-NP and FLAG-RUVBL1 by FLAG IP and −/+ RNase treatments. (D) Confirmation of NP-V5 and HA-RUVBL2 interaction by V5 IP and −/+ RNase treatments. (E) Reciprocal validation of protein-protein interaction between NP-V5 and HA-RUVBL2 by HA IP and −/+ RNase treatments. Monoclonal antibodies against HA, FLAG, and V5 were used to detect target proteins.