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. 2019 Apr 3;11(4):322. doi: 10.3390/v11040322

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Design of mosaic chikungunya virus (CHIKV) structural polyprotein sequence. (A) Neighbour-joining tree of full-length CHIKV structural proteins. (B) Intra-clade conservation (A–C) assessed by in-house sliding window algorithm. Conservation scaled between 0 and 1 with 0 being fully conserved within respective clades. Q1 cut-off for conservation of each clade indicated by coloured dashed line. (C) Inter-clade conservation (between A, B and C) across the three clades. Degree of conservation between either all three clades (blue) and only two clades (orange) indicated by cumulative conservation between 0 to −100.