Figure 4.

Cellular immune responses elicited by ChAdOx1 vaccines. (A) BALB/c mice (n = 6 per group) were immunised intramuscularly (i.m.) with a dose of 10⁸ I.U. of ChAdOx1 vaccines. Peripheral blood mononuclear cells (PBMCs) were cultured with a peptide pool containing the CHIKV structural antigens by ELISpot for IFNγ producing cells. (B) ELISpot from the same mice, using deconvolution of peptides spanning the C, E3, E2, 6k and E1 regions, respectively. Values represent the spot-forming cells (SFC) per million PBMCs. 20-mer peptides spanning the CHIKV structural polyprotein (10 µg/mL) were used for stimulation. Line colours and shapes represent mice vaccinated with each vaccine.