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CHIKV neutralisation assay. NT50 titres were assessed upon incubation of BHK-21 cells with CHIKV virus replicon particle-based (VRP) in the presence or absence of serially diluted sera from BALB/c mice, vaccinated with either ChAdOx1 sCHIKV (red line) or ChAdOx1 sCHIKV ∆C (green line) vaccines at 2-week post prime (A) and 1-week post-boost (B). NT50 titres were calculated using a non-linear fit of the log-dose versus response.
