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. 2019 Apr 21;11(4):568. doi: 10.3390/cancers11040568

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In apoptosis-sensitive, FL-TRAIL expressing cells, TRAIL appears in the supernatant owing to apoptosis, but not secretion (a) HEK293 cells were transfected with an empty plasmid (ctrl), a FL-TRAIL construct (FL) or an sTRAIL (sT) construct. The resulting medium supernatants were either taken as crude supernatants (c), centrifuged (s) or filtered through a 0.45 μm filter (f) before TRAIL levels were measured by ELISA (b) Apoptosis was measured in HEK293 and CHO cells that were transfected with an empty plasmid (ctrl), an FL-TRAIL construct (FL) or an sTRAIL construct (sT). (c) CHO cells were transfected with pCDNA3 constructs expressing FL-TRAIL (FL), sTRAIL (sT) or EGFP. The resulting protein lysates were subjected to western blotting. The membrane was then probed with a TRAIL antibody. (d) CHO cells were transfected with an empty plasmid (ctrl), a FL-TRAIL construct (FL) or an sTRAIL (sT) construct. The resulting medium supernatants were either taken as crude supernatants (c), centrifuged (s) or filtered through a 0.45 μm filter (f) before TRAIL levels were measured by ELISA. Values represent mean ± SE.