Table 1.
List of primers.
| Gene | Primer Sequence | Annealing temperature (°C) |
|---|---|---|
| hCx43 | F: CTTCACTACTTTTAAGCAAAAGAG R: TCCCTCCAGCAGTTGAG |
52 |
| hE-Cadherin | F: CAGAAAGTTTTCCACCAAAG R: AAATGTGAGCAATTCTGCTT |
58 |
| hZO-1 | F: CAGCCGGTCACGATCTCCT R: GTGATGGACGACACCAGCG |
58 |
| hGAPDH | F: TGGTGCTCAGTGTAGCCCAG R: GGACCTGACCTGCCGTCTAG |
58 |
| h18S | F: CAGCCACCCGAGATTGAGCA R: TAGTAGCGACGGGCGGTGTG |
58 |
| mGAPDH | F: CATGGCCTTCCGTGTTCCTA R: CCTGCTTCACCACCTTCTTGAT |
58 |
Cx43: Connexin43; F: Forward primer sequence; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; h: human; m: mouse; R: Reverse primer sequence; ZO-1: Zonula occludens 1.