Figure 5.

Infection study of the OB lysates collected from B. mori larvae co-infected with BmNPV and BmLV. On Day 1, fourth instar larvae were orally infected with BmLV-positive OBs. At 96 hpi, OBs were extracted from the hemolymph of three larvae (A–C) and dissolved in an alkaline solution. The OB lysates were inoculated onto BmVF cells. At 96 hpi, RNA was extracted and subjected to RT-PCR using primers for BmLV cp, BmNPV polh, and B. mori actin3. BmLV-negative and -positive OB lysates were used as controls. Size markers are indicated on the left side of the panel.