Skip to main content
. 2019 Apr 10;11(4):338. doi: 10.3390/v11040338

Figure 4.

Figure 4

Rescue effect of GAT medium on cell viability and ZIKV replication during MTX treatment. (A) Cell viability of Vero cells was studied by CTG reagent after MTX treatment with or without GAT medium. (B) GAT medium rescued the ZIKV replication from MTX treatment of the ZIKV-infected (H/PAN MOI 0.2) Vero cells. (C) Adenosine alone can save ZIKV replication during MTX treatment of the ZIKV-infected (H/PAN MOI 0.2) Vero cells. (D) Exogenous adenosine rescued cellular ATP levels, but thymidine and glycine could not rescue ATP levels during MTX treatment in Vero cells as measured by CTG assay. (E) Adenosine also rescued live-protease activity measured by CTF reagent during MTX treatment in Vero cells. (F,G) GAT media and adenosine were added to Vero cells and hNSCs to study determine their effect on host cell viability using CTG and CTF reagents. RLU = relative luminescence units. At least two independent replicates were performed. The error bars represent the standard error of the mean (SEM). One-way ANOVA, followed by Tukey’s multiple comparisons test, was used for statistical analysis. * p ≤ 0.05, **** p ≤ 0.0001, n.s. not significant.