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Western blot analysis of 569B OMVs and purified CT. Lanes 1 and 4: 569B OMVs and CT under non-boiled and non-reduced conditions. Lanes 2 and 5: 569B OMVs and CT under boiled and non-reduced conditions. Lanes 3 and 6: 569B OMVs and CT under boiled and reduced conditions. Two non-reducible anti-CT antibody-reactive bands were detected in 569B OMVs in each condition, but no band corresponding to the CTB5 pentamer was detected in the OMV sample.
