Figure 2.
Mean ± SEM of IL-10, IL-8, IL-6, IL-1β, and TNF-α productions by purified monocytes after stimulation with LPS 1 μg/mL (A), resiquimod 1 μg/mL (B) and zymosan 10 μg/mL (C) in men, women, and subjects with Klinefelter syndrome. Purified monocytes resuspended at 0.2 × 106 cells/mL in culture medium were incubated with the ligand for 24 h. The production of inflammatory cytokines was measured in the supernatant by flow cytometry. BD FACSDiva™ software version 6.1.2 for Windows was used to acquire the data and BD FCAP Array™ software version 1.0.1 for Windows was used to analyze the data. (*p < 0.05 and **p < 0.01 using one-way ANOVA for independent groups with n = 17).