Figure 4.
Mean ± SEM of phospho-ERK1/2 (A), phospho-P38-MAPK (B), and phospho-JNK1/2 (C) concentrations in purified monocytes after stimulation with LPS 10 μg/mL, resiquimod 1 μg/mL and zymosan 10 μg/mL in men, women, and subjects with Klinefelter syndrome. Purified monocytes resuspended at 10 × 106 cells/mL in culture medium were incubated with the ligand for 15 min. Concentrations of the phosphorylated kinases were measured in the cell lysate by flow cytometry. BD FACSDiva™ software version 6.1.2 for Windows was used to acquire the data and BD FCAP Array™ software version 1.0.1 for Windows was used to analyze the data.