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. 2019 May 9;10:1052. doi: 10.3389/fimmu.2019.01052

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Copyright © 2019 Lefèvre, Corazza, Valsamis, Delbaere, De Maertelaer, Duchateau and Casimir.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mean ± SEM of the median fluorescence intensity of phospho-ERK1/2, phospho-NF-κB-p65 and phospho-P38-MAPK in monocytes (A) and neutrophils (B), before and after stimulation of whole blood with LPS 10 μg/mL in men, women, and subjects with Klinefelter syndrome. Whole blood was stimulated with LPS at 10 μg/mL for 15 min. Reactions were stopped, and red blood cells removed. After washing, cells were permeabilized and mixed with fluorochrome-conjugated monoclonal antibodies against the phosphorylated kinases. The median fluorescence intensity of each fluorochrome was measured in each leukocyte population by flow cytometry. BD FACSDiva™ software version 6.1.2 for Windows was used to acquire the data and BD FCAP Array™ software version 1.0.1 for Windows was used to analyze the data.