. 2019 Mar 30;11(4):458. doi: 10.3390/cancers11040458

Table 1.

Centrifugation protocols for cfDNA/cfRNA analysis.

ID	Protocol	Specifications	Details	Temperature	Matrix
CP_Basic	Basic (Biobank UZA/UAntwerpen)	1. 10’ – 400 g		RT	Plasma
CP_{AdBasic_P}	Basic (adapted)	1. 10’ – 400 g 2. 1’ – max speed	2nd centrifugation step after storage at −80 °C	RT	Pellet
CP_AdBasic	Basic (adapted)	1. 10’ – 400 g 2. 1’ – max speed	2nd centrifugation step after storage at −80 °C	RT	Plasma
CP_Plat	Platelet	1. 20’ – 120 g 2. 20’ – 360 g 3. 5’ – 360 g	Platelets are washed with PBS in 3rd centrifugation step	RT	Plasma
CP_{Plat_P}	Platelet	1. 20’ – 120 g 2. 20’ – 360 g 3. 5’ – 360 g	Platelets are washed with PBS in 3rd centrifugation step	RT	Platelets
CP_Streck	Streck	1. 10’ – 1600 g 2. 10’ – 6000 g		RT	Plasma
CP_CEN	CEN	1. 10’ – 1900 g 2. 10’ – 16,000 g	Blood samples on ice immediately after blood collection	4 °C	Plasma
CP_AdCEN	CEN (adapted)	1. 10’ – 1900 g 2. 10’ – 16,000 g		RT	Plasma

A smooth braking profile was used in all centrifugation protocols to prevent disruption of the buffy coat layer. Plasma (and platelets) from each centrifugation protocol were snap frozen and stored at −80 °C. CEN: European Committee for Standardization; cfDNA: circulating cell-free DNA; cfRNA: circulating cell-free RNA; CP: centrifugation protocol; Platelets: only RNA isolation; RT: room temperature; 1: first; 2: second, and; 3: third centrifugation step, respectively.