Table 1.
Hepatic desaturases, oxidative stress and matrix metalloproteinases (MMPs) in methionine-choline-deficient (MCD) and Obese Zucker rats.
| Control MCD | MCD | p 1 | Lean Zucker | Obese Zucker | p 1 | p 1 | |
|---|---|---|---|---|---|---|---|
| D5D | 37.06 ± 4.3 | 8.89 ± 0.9 | <0.001 | 56.65 ± 12.5 | 27.09 ± 4.0 | <0.001 | # |
| D6D | 0.0076 ± 0.0012 | 0.0383 ± 0.0037 | <0.001 | n.d. | n.d. | n.d. | n.d. |
| D9-16D | 0.0565 ± 0.0082 | 0.0510 ± 0.0084 | <0.001 | 0.040 ± 0.0049 | 0.153 ± 0.021 | <0.001 | # |
| D9-18D | 1.249 ± 0.177 | 3.266 ± 0.273 | <0.001 | 0.437 ± 0.055 | 0.909 ± 0.054 | <0.001 | # |
| TBARS | 0.06 ± 0.003 | 0.34 ± 0.07 | <0.05 | 0.14 ± 0.01 | 0.11 ± 0.0006 | <0.05 | § |
| ROS | 26,677.3 ± 7691.3 | 3,384,750.2 ± 4844.4 | <0.05 | 27,671.0 ± 10,713.9 | 12,498.2 ± 987.4 | ns | § |
| GSH | 37.5 ± 1.6 | 16.1 ± 0.9 | <0.05 | 35.7 ± 2.0 | 31.2 ± 2.7 | ns | § |
| MMP-2 activity | 227.3 ± 17.3 | 397.1 ± 53.0 | <0.05 | 192.0 ± 5.8 | 139.7 ± 14.3 | ns | § |
| MMP-9 activity | 92.8 ± 12.5 | 163.3 ± 17.6 | <0.05 | n.d. | n.d. | ns | n.d. |
| MMP-2 protein | 1.8 ± 0.3 | 2.6 ± 0.5 | ns | 1.3 ± 0.2 | 1.1 ± 0.1 | ns | § |
| MMP-9 protein | 0.2 ± 0.007 | 0.5 ± 0.1 | ns | n.d. | n.d. | ns | n.d. |
1 ns: p value not significant; #: p < 0.001 or §: p < 0.05 MCD vs. Obese; n.d.: not detectable. TBARS, thiobarbituric acid reactive substances; ROS, reactive oxygen species; GSH, glutathione.