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. 2019 Apr 23;11(4):572. doi: 10.3390/cancers11040572

Figure 4.

Figure 4

Gap junction intracellular communication (GJIC) dysregulation in response to LMW PAHs is reversed with parthenolide treatment at 24 h in both C10 and E10 cells. (a) C10 cells were treated with the binary LMW PAH mixture (15 μM at 24 h; 1:1 ratio of 1-methylanthracene and fluoranthene) following a 1 h pre-incubation with these inhibitors (parthenolide, 10 μM; ACHP, 1 μM; CLI-095, 3 μM; glyburide, 50 μM; indomethacin, 1 μM). (b) Representative images of C10 cells following the SL/DT assay in these cells in response to the PAHs and parthenolide. All other inhibitor combinations can be found in Figure S2. (c) E10 cells were treated the same as C10 cells in a with these inhibitors parthenolide, ACHP, CLI-095, glyburide, indomethacin. (d) Representative images of E10 cells following the SL/DT assay in response to the PAHs and parthenolide. All other inhibitor combinations are in Figure S2. Experiments were repeated 3 times; n = 3 per treatment per experiment. PTL, parthenolide. * p < 0.05 for treatments compared to the control (DMSO) without inhibitors. + p < 0.05 for parthenolide + PAH treatment compared to PAH treatment without inhibitors. Magnification 100×.