Figure 8.

Recombinant TNF (rTNF) is not cytotoxic to C10 cells but does inhibit GJIC. (a) Cytotoxicity in C10 cells was assessed using the MTS assay at 24 h to determine any rTNF-induced toxicity or toxicity due to rTNF in the presence to 15 μM LMW PAH mixture (1:1 ratio of 1-methylanthracene and fluoranthene). No cytotoxicity was observed. (b) GJIC was measured using the SL/DT assay at both 4 and 24 h time points with rTNF alone. * p < 0.05 rTNF treatment compared to control; ⁺ p < 0.05 rTNF at 24 h compared to 4 h. rTNF, recombinant TNF; PAH, LMW PAH mixture described above. Experiments repeated 3 times with an n = 3 per treatment per experiment.