Skip to main content
. 2019 Apr 23;11(4):572. doi: 10.3390/cancers11040572

Figure 10.

Figure 10

rTNF-induced repression of CX43 protein expression at 24 h is enhanced following co-exposure to rTNF and the lower LMW PAH dose (8 μM). Both the high (15 μM) and low (8 μM) doses of the LMW PAH mixture (1:1 ratio of 1-methylanthracene and fluoranthene) were used to evaluate the effect of rTNF on CX43 protein expression using immunoblots. CX43 protein expression (upper blot) is normalized to bactin (lower blot). rTNF (1 ng/mL), LMW PAHs at both doses, and the combinations of both doses with rTNF are shown. The immunoblot depicted at the top is representative of 3 experiments with an n = 3 per treatment group per experiment. * p < 0.05 all treatments compared to the control (DMSO); + p < 0.05 rTNF compared to 15 μM PAH or rTNF plus 15 μM PAH; ** p < 0.05 8 μM PAH plus rTNF compared to either rTNF or 8 μM PAH alone. C, Cntl, control (DMSO); rTNF, rT, recombinant TNF (1 ng/mL); PAH, P, LMW PAH mixture; rT + P, rTNF plus PAH treatment.