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. 2019 May 9;11:108. doi: 10.3389/fnagi.2019.00108

FIGURE 2.

FIGURE 2

NMDAR is responsible for mediating the effects of Ca2+ on stimulating the production of p25 and phosphorylating tau at Ser396. (A–C) The brains of 9-month-old APP/PS1 Tg mice were collected after anesthesia and perfusion. (A) The NMDAR, p35/25 and p-tau levels and the total protein expression levels of tau were determined by western blotting using β-actin as an internal control. (B) The Ca2+ concentration was determined by atomic absorption spectroscopy. (C) The morphology of p-tau(Ser396) in APP/PS1 Tg mice was determined by immunohistochemistry. The scale bar represents 400 or 100 μm. (D) APP/PS1 Tg mice were treated with S(+)-ketamine (50 mg/kg) for 3 months (n = 6). The p35/25 and p-tau(Ser396) levels and the total protein expression levels of tau were determined by western blotting using β-actin as an internal control. The data represent the means ± S.E. of independent experiments. ∗∗p < 0.01 with respect to the WT mice.