Table 1.
The volume and concentration of qPCR system.
| Component | Volume | Final concentration |
|---|---|---|
| Go Taq qPCR Mastermix (2 × ) | 10 μl | 1 × |
| Forward primer (10 μM) | 1 μl | 0.5 μM |
| Reverse primer (10 μM) | 1 μl | 0.5 μM |
| cDNA template | 0.25 μl | |
| Nuclease-free water | 7.75 μl | |
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